Method for phthalates determination in milk.

OFFER from Romania, reference: TORO20180524001, valid from 06-06-2018 untill 09-06-2019

  • Title:
    Method for phthalates determination in milk.
  • Start date:
    6 juni 2018
  • End date:
    9 juni 2019
  • Summary:
    A Romanian research institute, active in the research and development of new methods in analytical chemistry, has developed a method for phthalates determination in milk, with useful applications in the food quality and safety field. Currently, the method is under development/laboratory testing. The institute is looking for partners such as food quality and safety laboratories to conclude commercial agreements with technical assistance (engineering and technical assistance).
  • Description:
    The Romanian research institute is specialized in the application of analytical chemistry in three main directions: Environment and Health, Instrumental Analytical Chemistry, Bioenergy and Biofuels. The institute has a remarkable endowment that allows approaching projects from research to stage prototype realization and technology transfer.

    Phthalates are organic substances and endocrine disruptors used as plasticizers in plastics. Scientific studies show that significant quantities of phthalates are present in the living environment, in food packaging (paper, plastics, cellophane, aluminum foil and jar lids) wherefrom, by migration, they may contaminate food and drink. Phthalates are fat soluble, so they can also be found in foods such as dairy products, eggs, chicken and pork, fish and oils. Therefore, through food ingestion, the human body may be exposed on:
    • long-term exposure to low levels of phthalates (chronic) causing male infertility, kidney and liver disorders, endocrine or potentially carcinogenic diseases;
    • short-term exposure to high levels of phthalates (acute) causing effects on the gastrointestinal system, dizziness or nausea.
    Based on these findings, a careful analysis of these endocrine disrupters is mandatory, in order to respect the food safety legislation. In this context, the Romanian institute has developed a method for phthalates determination in milk.

    As compared to other existing conventional methods on the phthalates determination such as Soxhlet extraction, the method developed by the Romanian institute uses “solvent-free” ultrasound extraction (USE) for extracting organic analytes from complex matrices and a low extraction time (approx. 90%).
    In order to determine the quantity and types of phthalates in milk, the method comprises the following steps: sample weighting, ultrasonic extraction with a solvent mixture, extract concentration, lipids removal, extraction in water, headspace solid-phase microextraction as well as identification and quantification of analytes by gas chromatography coupled with mass spectrometry (GC-MS).
    The method allows the determination of the following six phthalates in milk, at trace and ultra-trace level:
    • dimethyl phthalate (DMP),
    • diethyl phthalate (DEP),
    • di-n-butyl phthalate (DBP),
    • butyl benzyl phthalate (BBP),
    • di-(2-ethylhexyl) phthalate (DEHP) and
    • di-n-octyl phthalate (DOP).

    The method can be successfully used for monitoring the phthalates contamination of raw and commercialized pasteurized milk.

    The Romanian research institute is looking for partners abroad such as food quality and safety laboratories. The considered partnership refer to commercial agreements with technical assistance (engineering and technical assistance) in the method transfer and implementation on the partner's equipment, as well as, staff training.
Partner Sought
  • Type of Partnership Considered:
    Commercial agreement with technical assistance
  • Technical Specification or Expertise Sought:

    Type of partner sought: Agriculture and food analysis laboratories, active in the food quality and safety field. Under the commercial agreement with technical assistance, the sought partners should implement the method for monitoring of raw and commercialized pasteurized milk contamination, using appropriate equipment and qualified personnel. The Romanian research institute will support its partners with technical consulting and staff training.

    SME 11-50,SME <10,>500 MNE,251-500,SME 51-250,>500

  • Development stage:
    Under development/lab tested
  • IPR Status:
    Patents granted
  • Market keywords:
    Health food
    Agriculture, Forestry, Fishing, Animal Husbandry & Related Products
  • Technology keywords:
    Analytical Chemistry
    Detection and Analysis methods
  • NACE keywords:
    Other research and experimental development on natural sciences and engineering
  • Advantages and Innovations:
    Regarding the innovative character, the method developed by the Romanian institute:
    - uses “solvent-free” ultrasound extraction (USE) for leaching organic analytes from complex matrices,
    - requires a reduced extraction time (approx. 90%) as compared to conventional methods (e.g. Soxhlet extraction),
    - identifies and determines six phthalates by using gas chromatography-mass spectrometry (GC/MS).
    The elaborated method offers several advantages by using:
    - ultrasonic extraction,
    - the headspace solid-phase microextraction procedure - a rapid and simple extraction method requiring low time and quantities of organic solvents compared to conventional methods (Soxhlet extraction),
    - low detection limits.
  • Type and Size of Organisation:
    R&D Institution
  • Already Engaged in Trans-National Cooperation
  • Year Established:
  • Turnover:
  • Country of origin:
  • Languages spoken
    • Romanian
    • English